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A Guide to Absolute Counting on the Accuri C6 Flow Cytometer
03 Sep 2010: This technical note discusses features of the Accuri C6 Flow Cytometer® for simplifying cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. The microprocessor-controlled peristaltic pump system allows the sample volume pulled per run to be determined.
Accuri Cytometers, Inc.
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Recent articles: |
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In Vivo Monitoring of Fluorescently Labeled Cancer Cells
18 Aug 2010: This application poster demonstrates that both bioluminescence and fluorescence can be used to measure primary tumor growth and metastasis in vivo. Tumor progressions were monitored non-invasively and in real time using an enclosed imaging system containing a cooled CCD camera.
Caliper Life Sciences
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Use of Antibodies In Small Animal Molecular Imaging
18 Aug 2010: In vivo imaging enables the natural course of a disease or experimental model to be monitored. This application note discusses the use of fluorescently labeled primary antibodies for looking at protein expression in vivo imaging. The use of antibodies allows researchers to quickly determine if the test antibody begins to target undesired organs such as the kidneys or livers.
Carestream Molecular Imaging
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Characterization of Ion Channels in Stem Cell-derived Cardiomyocytes on An Automated Parallel Patch Clamp System
22 Jun 2010: In this application note Molecular Devices use the PatchXpress 7000A automated parallel patch clamp system is used to record Na, K and Ca currents from stem cell-derived mouse cardiomyocytes. The collaborative effort demonstrates that the combination of “ready-to-use” cardiomyocytes with an automated patch clamp system offers a powerful assay platform for fast assessment of compound efficacy and for safety profiling of lead compounds in a biologically relevant system.
Molecular Devices
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Expansion and Differentiation of H7 Human Embryonic Stem Cells in Chemically Defined Medium Using Corning® Synthemax™ Surface
21 Jun 2010: In this application note Corning Synthemax Surface is used to support long-term self-renewal and differentiation of H7 hESCs. Cells demonstrated a stable doubling time, high level of expression of hESC phenotypic markers (Oct4, SSEA4 and TRA-1-60) and normal karyotype. Corning Synthemax Surface also supported successful directed differentiation of H7 cells into functional cardiomyocytes, proving suitability of Corning Synthemax Surface for both expansion and differentiation of hESC culture.
Corning Life Sciences
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Human Oncostatin M is Functionally Equivalent to Mouse LIF in Supporting Mouse ES Cell Pluripotency & Germline Competency
21 Jun 2010: In this application note R&D Systems demonstrate human OSM is able to maintain ES cell pluripotency in a similar manner to mouse LIF, including long-term maintenance of pluripotency and the ability to differentiate in vitro, the ability to activate STAT3, and germline transmission competency. The study suggests that OSM may be an additional factor that plays a role in early embryonic development and that it may be used as an alternative to LIF in mouse ES cell culture.
R&D Systems, Inc.
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CO2 Incubator Sterilization method with 90ºC Moist Heat Decontamination
17 Jun 2010: In this application note, Esco validate the Swiftcon 90ºC moist heat decontamination cycle of the Celculture CO2 Incubator by performing decontamination tests on 5 bacterial suspension preparations, including Aspergillus niger and Bacillus subtilis. After decontamination, the microbial suspension discs were placed on tubes containing PBS which were assayed by culturing methods to quantitate Total Viable Count at each location.
Esco Micro Pte Ltd
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Microplate-Based Endotoxin Testing in GLP/GMP Environments with the VersaMax™ Absorbance Microplate Reader and Pyrochrome® Assay
07 Jun 2010: This application note describes a typical set-up for a standard curve in the Pyrochrome® Assay. The Pyrochrome® Assay together with the VersaMax Microplate Reader offers a sensitive and specific method for detecting endotoxin contamination in the production process of the pharmaceutical and medical device industries.
Molecular Devices
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Stem Cell-Derived Human Neurons in CNS Drug Discovery
07 Jun 2010: In this application note from Molecular Devices reproducible batches of human neurons are generated that will significantly improve and impact CNS drug discovery. The stem cell-derived neurons express all known markers typically associated with mature neurons and are functionally active. These neurons have been used in a recent screen to identify antagonists of a novel GPCR target implicated in schizophrenia.
Molecular Devices
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Cytotoxicity in Cells: Easy Determination Using Lonza ViaLight Plus and ToxiLight BioAssays on the SpectraMax L Microplate Luminometer
07 Jun 2010: The Lonza ViaLight® Plus and ToxiLight® Bioluminescent Cytotoxicity Assays exploit the cell characteristics of ATP levels and adenylate kinase release respectively, as cytotoxicity measures and enable detection using bioluminescent methods. This application note demonstrates that the SpectraMax L Microplate Luminometer and SoftMax Pro Software from Molecular Devices combine well with both assays providing a fast and sensitive combination of technologies in the field of cell death.
Molecular Devices
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